Dipeptide Diaminobutyroyl Benzylamide Diacetate for Anti-Wrinkle Formulations
If you formulate anti-aging serums or creams that target expression lines, this peptide belongs on your shortlist. Dipeptide Diaminobutyroyl Benzylamide Diacetate is a synthetic dipeptide modeled after waglerin-1, a component found in temple viper (Tropidolaemus wagleri) venom. It functions as a competitive antagonist at the muscular nicotinic acetylcholine receptor (mnAChR), producing a localized muscle-relaxing effect when applied topically. Unlike injectable neurotoxins, this peptide works through the stratum corneum at cosmetic use levels, making it suitable for daily-use products positioned as topical alternatives to professional treatments. We supply this ingredient from our warehouse in Valencia, Spain, with consistent lot-to-lot purity suitable for finished product manufacturing across the EU.
Mechanism of Action and Clinical Data
The peptide mimics the tripeptide sequence of waglerin-1 that binds to the alpha-1 subunit of the muscular nicotinic acetylcholine receptor. By acting as a reversible competitive antagonist at this receptor, it inhibits sodium ion influx, reducing the frequency and intensity of muscle micro-contractions beneath the skin surface. This mechanism is distinct from botulinum toxin (which blocks acetylcholine release presynaptically) and from hexapeptides like Acetyl Hexapeptide-3 (which target SNARE complex assembly).
In a controlled 28-day in-vivo study using a 4% active solution applied twice daily, panelists showed a 52% reduction in wrinkle depth and a 60% reduction in wrinkle roughness measured by skin profilometry. Visible smoothing was reported as early as day 14. In-vitro receptor binding assays confirm an IC50 in the low micromolar range against the mnAChR, supporting a genuine pharmacological mechanism rather than a film-forming or hydration artifact. The effect is reversible and dose-dependent, meaning formulators can tune intensity by adjusting concentration within the recommended 1-4% range of active solution.
Formulation Guide
Use Dipeptide Diaminobutyroyl Benzylamide Diacetate at 1-4% of the active solution in your final formulation. The commercial raw material is typically standardized to approximately 50 ppm peptide content in a glycerin or butylene glycol carrier. The peptide is water-soluble and compatible with glycols, so it incorporates easily into aqueous phases of emulsions, serums, and toners.
Add to your formulation below 40°C during the cool-down phase. The optimal pH window is 5.0 to 7.0. Avoid formulations with a final pH below 3.0, as strong acid conditions can hydrolyze the peptide bond. It is compatible with most cosmetic ingredients including hyaluronic acid, niacinamide, and common preservative systems. For maximum wrinkle-targeting effect, combine with Copper Tripeptide-1 (collagen remodeling) or Acetyl Tetrapeptide-9 (dermal matrix support) in layered or combined formulations.
Frequently Asked Questions
What is the difference between this peptide and Acetyl Hexapeptide-3 for expression lines?
They target different points in the neuromuscular pathway. Acetyl Hexapeptide-3 (Argireline) inhibits SNARE complex formation to reduce vesicle docking and acetylcholine release. Dipeptide Diaminobutyroyl Benzylamide Diacetate works post-synaptically by blocking the nicotinic receptor itself. In practice, the two can be combined for a dual-mechanism approach, though each is effective independently at its recommended use level.
Can I use this in oil-based formulations?
The peptide solution is water-soluble and not directly miscible with oils. In W/O emulsions, incorporate it into the internal aqueous phase. For anhydrous products, you would need to encapsulate or use a solubilizer, though aqueous serums and O/W emulsions are the most straightforward vehicle types.
Is there a maximum recommended concentration?
The supplier-recommended maximum is 4% of the active solution. Going higher does not proportionally increase efficacy based on available dose-response data, and it increases formula cost without clear benefit. Most commercial finished products use 2-4%.
Does it require cold-chain shipping?
No. The peptide solution is stable at room temperature (below 25°C) for its stated shelf life. Store away from direct sunlight. For long-term storage beyond 12 months, refrigeration at 2-8°C is recommended but not mandatory for normal inventory turnover.
What claims can I support on-pack with this ingredient?
Based on the available study data, supportable claims include “reduces appearance of expression lines,” “smooths wrinkle depth,” and “visible results in 28 days.” Avoid drug-like claims about muscle paralysis or comparing directly to botulinum toxin in consumer-facing materials, per EU cosmetics regulation guidance.
Specifications
| Parameter | Value |
|---|---|
| INCI Name | Dipeptide Diaminobutyroyl Benzylamide Diacetate |
| CAS Number | 823202-99-9 |
| Molecular Weight | 472.54 g/mol |
| Purity | ≥95% (HPLC) |
| Solubility | Water, glycols |
| Recommended pH | 5.0 – 7.0 |
| Use Level | 1-4% active solution |
| Storage | Below 25°C, protect from light |
| Certifications | CoA per lot, EU cosmetic grade |
Related Products
Formulators working with expression-line peptides often pair with these actives:
- Copper Tripeptide-1 – collagen synthesis and dermal remodeling peptide
- Acetyl Tetrapeptide-9 – lumican stimulator for dermal matrix density
Order and Supply
TCS Peptides supplies Dipeptide Diaminobutyroyl Benzylamide Diacetate from our warehouse in Valencia, Spain. Standard delivery within the EU takes 5-7 business days. We provide a Certificate of Analysis with every lot shipped.
To request pricing or place an order, contact us by email at info@tcspeptides.com or via WhatsApp (+34 672929362). Our technical team can assist with formulation compatibility questions for your specific application.